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应用原位杂交技术分析ZNF216基因在成年小鼠各主要组织中的表达模式(英文) 被引量：1

Analysis of ZNF216 expression in adult mouse multiple tissues using in situ hybridization
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摘要 目的 :获得ZNF2 16基因的表达模式。方法 :体外转录方法合成地高辛标记的cRNA探针 ,利用原位杂交技术检测ZNF2 16基因在成年小鼠各主要组织中的表达模式。结果 :在小鼠脑、心脏、骨骼肌和睾丸细胞内可检测到很强的ZNF2 16的杂交信号 ,在肾脏细胞内也可检测到杂交信号 ,但在脾脏和肝脏细胞内仅检测到少许杂交信号和未能检测到杂交信号。结论 :ZNF2 16基因表达于几种组织细胞内 ,具有一定的组织特异性。 Objective To investigate the expression pattern of ZNF216 in adult mouse multiple tissues. Methods Digoxigenin-labeled cRNA probes were synthesized in vitro, and the expression pattern of gene ZNF216 in adult mouse multiple tissues was analyzed using in situ hybridization. Results The strong ZNF216 mRNA hybridization signal was observed in brain, skeletal muscle, heart and testis, moderate one in kidney, and weak one in spleen and vacant one in liver. Conclusion ZNF216 expresses differently in adult mouse multiple tissues.
作者 顾香芳 甘光明 王芳 谢维
机构地区 东南大学医学院遗传与发育生物学系
出处 《东南大学学报（医学版）》 CAS 2005年第1期1-4,共4页 Journal of Southeast University(Medical Science Edition)
基金 Thenationalawardforexcellentresearchandteachingfromtheministryofeducation(2 0 0 1 1 82 )
关键词 成年小鼠 原位杂交技术 基因 杂交信号 CRNA探针 肝脏细胞 肾脏细胞 表达模式 组织特异性 地高辛标记 zinc fingers/genetics ZNF216 in situ hybridization,fluorescence gene expression mice
分类号 R329.28 [医药卫生—人体解剖和组织胚胎学] R338.1 [医药卫生—人体生理学]
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同被引文献17

  • 1谢维.MHC基因多态性和肿瘤的发生[J].现代免疫学,2004,24(6):441-444. 被引量：8
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东南大学学报（医学版）
2005年 第1期

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