氨基胍与葛根素对糖尿病大鼠肾脏结缔组织生长因子表达的影响

Effects of aminoguandine and puerarin on expression of connective tissue growth factor in kidneys of diabetic rats

目的 :探讨结缔组织生长因子 (CTGF)在糖尿病肾病发病中的作用及葛根素 (Pue)和氨基胍 (AG)对其表达的干预作用。方法 :将 60只雄SD大鼠随机均分成正常对照组、糖尿病组、氨基胍组和葛根素组 ,腹腔注射链脲佐菌素建立糖尿病大鼠模型。氨基胍组在建模基础上喂食氨基胍进行干预 ,葛根素组采用腹腔注射葛根素干预。逆转录聚合酶链反应和免疫组织化学法观察各组肾组织中CTGF基因和蛋白的表达。结果 :糖尿病大鼠肾皮质CTGF GAPDHmRNA之值为 0 .44± 0 .0 3 ,明显高于葛根素组的 0 .2 2± 0 .0 2和氨基胍组的 0 .2 3± 0 .0 2 (均P <0 .0 1) ,后两组又高于正常对照组的 0 .16± 0 .0 2 (均P <0 .0 1)。免疫组织化学法显示 ,正常对照组大鼠肾小球CTGF阴性 ,葛根素组和氨基胍组染色相近 ,强于正常对照组 ,但显著弱于糖尿病组。结论 :糖尿病大鼠肾皮质CTGF表达增强 ,可能部分参与糖尿病肾病的发生发展过程 ,氨基胍和葛根素降低CTGF表达的作用可能是其肾脏保护的机理之一。

Objective To investigate whether connective tissue growth factor (CTGF) is involved in pathogenesis of diabetic nephropathy and determine consequently whether aminoguanidine (AG) and puerarin(Pue)can attenuate enhanced CTGF expression in kidneys of streptozotocininduced diabetic rats. Methods Expression of mRNA and protein of CTGF in kidney of diabetic rat were quantified by RTPCR with GAPDH as an internal standard and histochemistry demonstrated by monoclonal antibodies against CTGF,respectively. Results It was shown that expression of CTGF/GAPDH mRNA ratio was markedly enhanced in kidney of diabetic rat(0.44±0.03) compared with control group(0.16±0.02). After treatment,AG group ( 0.23±0.02) and Pue group (0.22±0.02) lowed down CTGF expression in kidneys of streptozotocin induced diabetic rats,but still with higher expression level than control group. Immunochemistry showed that positive particles per glomerular increased in diabetic group compared with control group,which could be partly decreased by treatment of AG and Pue. Conclusion The results suggest that CTGF plays a role in diabetic nephropathy. Pue and AG might be of clinical significance in preventing diabetic nephropathy from developing by alleviating increased expression of CTGF.