猕猴早期内毒素血症肾脏内选择素的变化

Changes of selectin in kidney of macaque with early-phase endotoxemia

目的 :研究选择素在猕猴早期内毒素血症肾脏内变化。方法 :将 11只猕猴随机分成 2组 :对照组 5只 ,静脉注射生理盐水 ;内毒素组 6只 ,静脉注射脂多糖 ,剂量为 2 .8mg·kg- 1 。内毒素攻击后 12 0min ,处死动物 ,光镜观察肾脏标本及行选择素免疫组织化学 (免疫组化 )检测。结果 :组织学显示 ,肾髓质、皮质结构完整 ,肾小球结构完好。免疫组化显示 ,内毒素组动物中 ,P 选择素在肾小球血管内和肾小管周围均有表达 ,有血细胞沉积 ;在肾小球的血管内见血细胞聚集附着管壁 ,L 选择素呈丝网状分布于血细胞之间。对照组无明显病变。结论 :在内毒素休克早期肾脏内发生损伤 ,选择素介导的血管损伤发挥了重要的作用。

Objective To evaluate the effect of selectin in the kidney of macaque with early-phase endotoxemia. Methods Eleven macaques were randomly divided into two groups, namely, control group (Co group, n=5) and endotoxic group (En group, n=6). The animals of the Co group were injected with 1ml·kg -1 normal saline and the subjects of the En group were received a dose of 2.8mg·kg -1 lipopolysaccharides (LPS) i.v. 120 min after LPS challenging, histological detection and immunohistochemical assay of P-selectin and L-selectin in macaque kidney were carried out. Results Renal medullae and cortex were intact, and so was glomerulus under microscope in LPS animals. By immunohistochemical assay in LPS macaques, P-selectin in glomerular vessels and tissues around tubules was observed. Aggregation of blood cells associated with P-selectin precipitation was revealed in vessels. By L-selectin immunohistochemical staining, congregated blood cells attached to the vascular wall, and blood cells were surround by reticular L-selectin in the En group. No pathological changes were observed in the control group. Conclusion Vascular damages in kidney induced by selectin appear in the early phase of endotoxic shock and play an important role for the pathogenesis of kidney.