月桂氮酮抗眼科丝状真菌活性的体外研究

Antifungal activity of laurocapram against ocular pathogenic filamentous fungi in vitro

目的 评价高效皮肤促渗剂月桂氮酮 (氮酮 )体外抗眼科丝状真菌活性。方法 用微量液基稀释法测定氮酮对 4株眼科常见致病丝状真菌 (串珠镰刀菌、烟曲霉菌、黄曲霉菌及黑曲霉菌 )的最低抑菌浓度 (MIC)。结果 氮酮对串珠镰刀菌、烟曲霉菌、黄曲霉菌及黑曲霉菌的MIC分别为 0 . 0 0 31,0 . 0 0 6 3,0 . 0 0 31及 0 . 0 12 5 μL·mL-1。结论 氮酮对 4株眼科常见致病丝状真菌有明显的体外抗菌活性 ,说明抗真菌滴眼剂的配方中加入氮酮除可增加抗真菌药的角膜透性外 ,适当浓度的氮酮与抗真菌药可能还有协同抗真菌作用。

OBJECTIVE To evaluate the antifungal activities of penetration enhancer laurocapram(Azone) against ocular pathogenic filamentous fungi in vitro.METHODS The minimum inhibitory concentration (MIC) of Azone against 4 strains of ocular pathogenic filamentous fungi (Fusarium moniliforme, Aspergillus flavus, Aspergillus fumigatus, and Aspegillus niger) were determined by broth microdilution method.RESULTS MICs of Azone were 0.003 1 μL·mL -1 against Fusarium monilifore and Aspergillus flavus, 0.006 3 μL·mL -1 against Aspergillus fumigatus, 0.012 5 μL·mL -1 against Aspergillus niger, respectively.CONCLUSION Azone was active against ocular pathogenic filamentous fungi in vitro. It suggested that Azone incorporated into the ophthalmic formulations of antifungal drugs had two functions. One was promoting the cornea absorption of antifungal drugs,and the other was cooperating antifungal activities with antifungal drugs. The two functions of Azone will increase therapeutic action of antifungal agents.