快速减压对小鼠血清细胞因子水平的影响

Effects of rapid decompression on serum level of cytokines in mice

目的 观察快速减压对小鼠血清IL 1β、IL 6和IL 10水平的影响 ,以探讨细胞因子在减压病发病机制中的作用。方法 2 1只小鼠随机分为快速减压组 (14只 )和正常对照组 (7只 )。快速减压组经60 0kPa压缩空气暴露后 ,用 1min快速减压至常压 ,用酶联免疫吸附法检测减压后快速减压组小鼠及正常对照组小鼠血清IL 1β、IL 6和IL 10水平。结果 快速减压组小鼠减压后 1h时血清IL 1β和IL 6水平与正常对照组相比无显著变化 ,3h组血清IL 1β和IL 6水平显著高于正常对照组 (均P <0 .0 5 )。快速减压组小鼠 1h和 3h时血清IL 10水平与正常对照组相比 ,差异均无统计学意义。结论 快速减压可导致小鼠血清IL 1β、IL 6水平显著升高 ,诱发促炎症介质和抗炎症介质之间的失衡。

Objective To observe the effects of rapid decompression on serum level of IL-1β,IL- 6 and IL-10 in mice and investigate the role of cytokines in the pathogenesis of decompression sickness. Methods Twenty-one mice were randomly divided into rapid decompression group (14 mice) and normal control group (7 mice). Mice in the rapid decompression group were exposed to 600kPa compressed air for 60 minute,which was then rapidly decompressed to normal pressure in one minute. The serum level of IL-1β,IL- 6 and IL-10 was measured by enzyme-linked immuno-sorbent assay in mice in rapid decompression group after decompression and in normal control group. Results There were no significant changes in the serum level of IL-1β,IL- 6 at the 1st hour after decompression in the rapid decompression group when compared to that of and the normal control group. The serum level of IL-1β,IL- 6 in rapid decompression group at the 3rd hour after decompression was significantly higher than that of the normal control group(P<0.05).There were no significant difference between the rapid decompression and normal control groups with regard to the serum level of IL-10. Conclusion Rapid decompression can significantly increase the serum level of IL-1βand IL- 6 in mice, then induce the unbalance between inflammatory and anti-inflammatory mediators.