摘要
本研究利用24对SSR引物和36个ISSR引物,分析33份来源于亚洲10个国家的香稻品种的遗传多样性.分别获得93条和181条多态性片段,每个SSR座位可检测3～8个等位基因,平均为4.23个;每个ISSR引物可检测3～8个多态性位点,平均为5.03个.根据SSR和ISSR标记计算的品种间遗传相似系数分别在0.294～0.884之间和0.595～0.867之间.聚类分析表明,利用两种标记所得的聚类结果基本上一致,与品种所处的3种气候类型变化基本相符.进一步证实SSR和ISSR标记是研究水稻种质资源分类有效的工具.
The objective of the present study was to make use of efficient molecular marker systems to reveal genetic diversity among 33 fragrance cultivars from 10 countries in Asia. The cultivar groups were analyzed by using 24 pairs of SSRs and 36 ISSR primers. A total of 93 and 181 polymorphic bands were found in 33 varieties respectively each kind of markers. The average number of alleles per SSR locus was 4.23 with a range from 3 to 8. The average number of polymorphism bands per ISSR primer was 5.03 with a range from 3 to 8. Genetic similarities among the 33 rice accessions ranged from 0.294 to 0.884 and from 0.595 to 0.867, respectively by means of SSR and ISSR markers. The clustering analysis based on ISSR data is very identical to that based on SSR data basically. The results were consistent with three types of climate which the cultivars come from. Consequently, SSR and ISSR markers could be used as a useful tool for measuring genetic diversity.
出处
《分子植物育种》
CAS
CSCD
2005年第1期37-42,共6页
Molecular Plant Breeding
