摘要
番茄是世界上最主要的蔬菜作物之一,叶霉病(Cladosporium fulvum Cooke)的发生和蔓延使保护地番茄生产受到严重影响,培育抗叶霉病的番茄品种是控制该病害最经济有效的方法.本研究根据Cf-5的基因序列设计特异扩增引物,以7个含有不同叶霉病抗病基因的品种为试材,扩增Cf-5基因2 558～3 523bp之间的单拷贝片段,7个材料均获得了约0.96Kb的特异扩增片段.用限制性内切酶Taq I对该片段进行酶切,含Cf-5基因的材料产生了一条256bp的特异片段,而不含Cf-5基因的材料产生一条225bp的特异片段.从而建立了Cf-5基因的共显性CAPS标记.这一研究结果为Cf-5基因的分子标记辅助育种奠定了良好基础.
Tomato is one of the most important vegetable crops in the world. Tomato leaf mould (Cladosporium fulvum Cooke) has seriously threatened the tomato production under protected cultivation. Genetic improvement is the most economical ways to reducing the losses caused by leaf mould. In this paper, PCR primers were designed based on the sequence of a single copy from 2 558bp to 3 523bp in Cf-5 gene. A 0.96Kb fragment was amplified in all of 7 differential hosts, and then the PCR products were digested with restriction enzyme TaqⅠ. A fragment of 256bp was detected in the host variety with Cf-5 gene, and a fragment of 225bp in other 6 varieties. CAPS marker was developed based on the polymorphism of restriction sites. This result will provide a useful tool for molecular assistant selection of leaf mould resistance in tomato breeding.
出处
《分子植物育种》
CAS
CSCD
2005年第1期57-60,共4页
Molecular Plant Breeding
基金
国家高技术研究发展计划(863计划)(批准号:2002AA244021)项目
北京市科技计划项目“蔬菜种质改良中心建设及产业化开发”(合同编号:H022020130130)的资助。
