摘要
ISSR(inter-simplesequencerepeat)标记技术是在SSR基础上发展起来的一项新的标记技术,由于其引物设计比SSR简单,不需要知道SSR两端的碱基序列,多态性高,重复性好,能够提供更多的信息,已在多种动植物的种质鉴定、遗传作图、基因定位、遗传多样性等研究方面得到应用。本文对ISSR技术的特点、实验操作及其在作物遗传育种领域的应用进行了概述。
Recently, inter-simple sequence repeat (ISSR) markers have emerged as an alternative system with reliability and advantages of microsatellites(SSR). ISSR primer can be designed without the knowledge about microsatellite regions, and its design is easer than that of SSR. ISSR marker has good polymorphism and repetition, and can provide more information than others. Its technique has been widely used in the studies of germplasm identification, genetic mapping, gene tagging, genetic diversity in animal and plant. This paper emphasizes on the characteristics and procedures of ISSR and its application in crop genetics and breeding.
出处
《分子植物育种》
CAS
CSCD
2005年第1期123-127,共5页
Molecular Plant Breeding
