基质金属蛋白酶及其组织抑制剂在涎腺多形性腺瘤生物学行为中的意义

The expression of matrix metalloproteinases and their tissue inhibitors in pleomorphic adenoma

目的 研究涎腺多形性腺瘤中基质金属蛋白酶 (MMP) 2、9及其组织抑制剂 (TIMP) 1、2的表达变化与其生物学行为的关系。方法 用免疫组化SP法和明胶酶谱法分别检测 9例普通型和14例生长活跃型多形性腺瘤中MMP 2、9及TIMP 1、2的阳性表达及细胞定位 ,分析其中酶原与活性酶的含量比例。结果 MMP 2及MMP 2 /TIMP 1、MMP 2 /TIMP 2的比值在生长活跃型多形性腺瘤中的表达高于普通型 ,活性MMP 2、MMP 9酶原和活性酶在生长活跃型多形性腺瘤中的表达明显高于普通型 ,涎腺良性肿瘤与普通型多形性腺瘤间、涎腺恶性肿瘤与生长活跃型多形性腺瘤间差异无统计学意义。结论 涎腺生长活跃型多形性腺瘤与涎腺恶性肿瘤有相似的MMP 2、9和TIMP 1、2表达特征 ,而普通型多形性腺瘤中MMP 2、9和TIMP 1、2的表达与涎腺良性肿瘤相近。检测MMPs。

Objective To examine the expression of MMPs and TIMPs in pleomorphic adenoma of salivary gland and to investigate the relationship between the expression and the biological behaviour of the tumor.Methods Twenty three cases of pleomorphic adenoma were divided into active type and common type according to their biological behavior. Immunohistochemistry for MMP 2、MMP 9、TIMP 1、TIMP 2 and gelatin zymography analysis were performed in these 23 cases and in 6 malignant and 6 benign salivary gland tumors.Results The immunoreactivity of MMP 2 protein and MMP 2/TIMP 1、2 ratio were significantly higher in active pleomorphic adenoma than in common pleomorphic adenoma ( P =0 028、 P =0 009、 P =0 045). The expression of active MMP 2、proMMP 9 and active MMP 9 were significantly higher in active pleomorphic adenoma than in common pleomorphic adenoma( P =0 034 、 P =0 021、 P =0 001). There was no significant difference in expression of MMP 2、MMP 9、TIMP 1 and TIMP 2 between the salivary malignant tumor and active pleomorphic adenoma , also between the salivary benign tumor and common pleomorphic adenoma .Conclusions The expression of MMPs and TIMPs in active pleomorphic adenoma is similar to that in salivary carcinomas,and the expression in common pleomorphic adenoma also resembled to that in salivary adenoma . The expression of MMP 2、9 and TIMP 1、2 is related to the biological behavior of pleomorphic adenoma of salivary gland