手工微柱凝胶免疫检验法在ABO、RhD血型抗原鉴定中的应用

Evaluation of manual microcolumn gel immune method for detecting antigens of blood groups of ABO and RhD

目的 :探讨手工微柱凝胶免疫检验法在ABO、RhD血型抗原鉴定中的应用。方法 :分别取 5 0份不同科室、病种、性别的患者新鲜抗凝、非抗凝血标本为Ⅰ、Ⅱ组 ;取 5 0份库存 15天以上有效期内的库存血标本为Ⅲ组 ;取 5 0份保存 3～ 7天的非新鲜血标本 (可能被污染 )为Ⅳ组 ,同时用手工微柱凝胶法和试管法进行ABO、RhD血型抗原鉴定 ,比较反应结果及反应强度。结果 :Ⅰ、Ⅱ、Ⅲ组标本在ABO、RhD抗原鉴定中 ,手工微柱凝胶法与试管法所得结果完全相符 (P >0 .0 5 ) ,反应强度微柱凝胶法高于试管法 ;Ⅳ组标本在ABO、RhD血型抗原鉴定时微柱凝胶法检出 2例假阳性标本 ,其余 4 8份标本两法鉴定结果完全相符 (P >0 .0 5 )。结论 :手工微柱凝胶法可代替试管法常规用于ABO、RhD血型抗原鉴定。

Objective:To evaluate manual microcolumn gel immune method in detecting antigens of blood groups of ABO and RhD.Methods:Fifty samples of fresh anticoagulant blood,50 samples of fresh non-anticoagulant blood,50 samples of blood stocked for 15 days,but within valid period and 50 samples of non-fresh blood stocked for 3-5 days(possibly contaminated blood),which were collected from different departments,diseases and genders of patients,were classified into Ⅰ,Ⅱ,Ⅲ and Ⅳ groups respectively.Blood antigens of ABO,RhD in all samples were assayed by manual microcolumn gel immune method and tube method.The response result and response intension were compared.Results:The result by manual microcolumn gel immune method in Ⅰ,Ⅱ and Ⅲ groups was identical with that by tube method in detecting antigens of blood type of ABO and RhD(P>0.05),but the response intension was stronger by manual microcolumn gel immune method than that by tube method.Two improper positive samples in Ⅳ group were found by manual microcolumn gel immune method and the other 48 samples had the same results by two methods(P>0.05).Conclusions:Manual microcolumn gel immune method can substitute tube method for detecting antigens of blood groups of ABO and RhD.