5-羟色胺对皮层扩展性抑制的影响及大川芎丸的干预作用

Influence of 5-HT on Cortical Spreading Depression and the Pharmacological Effect of Dachuanxiong Pill

目的 :观察 5 羟色胺 (5 HT)对皮层扩展性抑制 (CSD)的增强作用以及大川芎丸的干预作用。方法 :利用KCL诱发CSD ,采用玻璃微电极记录CSD的电生理变化。腹腔注射 5 羟基色胺酸 (5 HTP)造成高 5 HT状态 ,以观察 5 HT对CSD的增强作用 ;连续灌胃大川芎丸 7d后、以观察该药对正常状态和高 5 HT状态下CSD的干预作用。结果 :5 HT可以明显延长CSD的持续时间。大川芎丸可以降低正常状态CSD的幅度和出现个数 ;缩短高 5 HT状态下的CSD持续时间。结论 :5 HT对CSD具有明显的增强作用 ;大川芎丸可以抑制CSD ,可能是其治疗偏头痛的作用机制之一。

Objective:To observe the enhancement effect of 5 HT and the pharmacological effect of Dachuanxiong Pill to CSD.Method:Induced CSD with KCL to rats and recorded the electrophysiological change of them with glass microelectrode.Observe the enhancement effect of 5 HT to CSD in hyperserotonin state after intraperitoneal injection with 5 HTP to rats; Observe the phamacological effect to CSD in normal and hyperserotonin state after intragastric administration continuously with Dachuanxiong Pill to rats for seven days.Result: 5 HT can significantly prolonged the latent period and duration of CSD.The pharmacological effect of Dachuanxiong Pill exhibited the potency of decreasing normal range and occurrence time of CSD; Reducing the episode times of CSD in hyperserotonin state.Conclusion:5 HT has significantly enhancement effect to CSD;Dachuanxiong Pill has the potency of inhibit the occurrence time of CSD.So this maybe the treatment mechanism of migraine.