白细胞介素4和受体基因多态性与儿童变应性哮喘的相关分析

Polymorphisms in IL-4 and IL-4R genes in children with allergic asthma

目的探讨白细胞介素4(IL-4)及受体(IL-4R)基因多态性与儿童变应性哮喘易感性及与血浆总IgE的关系。方法采用聚合酶链反应-限制性酶切多态性方法检测IL-4基因启动子区-589位和IL-4Rα亚单位Q576R两位点基因多态性,并观察对血浆总IgE的影响。结果(1)白细胞介素-4基因-589基因多态性与儿童支气管哮喘无相关关系;(2)IL-4受体α亚单位RR基因型和R576等位基因频率在儿童支气管哮喘与对照组相比差异显著(P<001,P<001),且R576与高IgE相关。结论这些数据表明IL-4Rα亚单位R576是中国汉族变应性哮喘患儿的危险因子,且与高IgE相关。

AIM: To determine whether interleukin 4 and interleukin 4 receptor α chain are associated with allergic asthma in children and to study the impact of such polymorphism upon plasma IgE.METHODS: Two polymorphism sites of IL-4 and IL-4R were studied by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).RESULTS: (1)The results showed that the IL-4 promoter-589 was not associated with children allergic asthma, however, the IL-4R α chain 576RR genotype and R allele were significantly increased in the subjects with asthma in children compared with age-matched control subjects (χ2=11.84, P<0.01; χ2=13.03, P<0.01). The IL-4R α chain 576RR genotype was associated with higher plasma IgE. CONCLUSION: These date suggest that the IL-4R α chain R576 is a risk factor of allergic asthma in children in Chinese, and is also related with higher plasma IgE level. [