摘要
目的研究神经生长抑制因子Nogo-AmRNA在大鼠缺血性脑梗塞脑内不同时点表达的变化,探讨Nogo-A基因在缺血性脑梗塞时神经再生的作用。方法建立缺血性脑梗塞(MCAO)模型,采用原位杂交法结合免疫组化技术,检测80只在不同时期缺血性脑梗塞大鼠脑内Nogo-A的表达量。结果原位杂交法显示Nogo-AmRNA在脑梗塞后3d下降,7d后上升,2周达到高峰,第3、4周保持第2周水平;免疫组化显示Nogo-A含量在脑梗塞后3d下降,7d后上升,3周达到高峰,第4周保持第3周水平。结论脑内Nogo-A表达在脑梗塞急性期(3d)变化不明显,而在1周后较为明显,表明在脑梗塞后期Nogo-A表达有明显升高,它可能是造成成年哺乳动物损伤后神经再生障碍的重要原因之一。
AIM: To investigate the dynamic changes of Nogo-A expression in ischemic infarct brain in rats. METHODS: The model of middle cerebral artery occlusion (MCAO) in 80 rats was established and expression of Nogo-A mRNA was measured by immunohistochemistry and hybridization. RESULTS: In the brain of MCAO rats, Nogo-A mRNA expression was decreased at the third day and increased significantly at the 7th day, and reached high level at the 21th day, then remained the high level to the 28th day. Nogo-A protein expression showed the same results. CONCLUSION: Expression of Nogo-A did not change in the early stage of MCAO, but increased significantly in the late stage of MCAO, suggesting that Nogo-A expression may play an important role in the nerve regeneration of brain ischemic injury. [
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2005年第1期143-147,共5页
Chinese Journal of Pathophysiology
