次声作用后血浆NO、ET-1、SOD、MDA水平的变化

Plasma levels of endothelin-1, nitric oxide, malondialdehyde and superoxide dismutase in rats exposed to infrasound

目的测定8Hz、130dB次声不同时间暴露后大鼠血浆一氧化氮(NO)、内皮素(ET-1)、SOD、MDA水平的变化。方法用8Hz、130dB的次声连续作用大鼠1、7、14、21和28d,每天2h,测定大鼠血浆NO、ET-1、SOD、MDA水平。结果在暴露期间,7、14d时大鼠血浆NO含量显著最低(P<001),1d、21d和28d时正常(P>005);大鼠血浆ET-1含量均明显升高(P<001),7d时升高最多,14d时升高最少;大鼠血浆SOD活性明显降低(P<001);大鼠血浆MDA水平明显升高(P<001)。结论次声可引起大鼠血浆NO、ET-1、SOD、MDA水平的变化,发生的改变与次声暴露时间有关。

AIM: To observe the changes of NO, ET-1, SOD and MDA levels in plasma of rats exposed to infrasound. METHODS: Using infrasound (frequency: 8 Hz; sound pressure level:130 dB), the rats were exposed for 1 d, 7 d, 14 d, 21 d and 28 d, 2 h daily, then the levels of NO, ET-1, SOD and MDA were measured after exposure. RESULTS: The changes of NO levels in plasma significantly declined at 7 d and 14 d (P<0.01), then 1, 21 and 28 d normally (P>0.05). The changes of ET-1 levels in all groups in plasma were significantly increased (P<0.01), mostly at 7 d, least at 14 d. The changes of SOD activity in all groups in plasma were significantly declined (~P<0.01 ). The changes of MDA levels in all groups in plasma were significantly increased (P<0.01). CONCLUSIONS: Infrasouns induces changes of NO, ET-1, SOD and MDA in rat plasma, and it depends on infrasound exposure time. [