PF4与BMP-2m在γ-射线照射后造血损伤治疗中作用的比较

Comparative study of effect of PF4 and BMP-2m on hematopoietic damage in mice treated with γ-ray

目的 :比较PF4 ,BMP 2m及二者联用对大剂量射线照射后造血损伤的作用 ,比较二者的作用效果及有无协同作用 .方法 :BALB/c小鼠 36只 ,随机分成正常对照组、照射对照组、PF4 ,BMP 2m ,PF4 +BMP 2m联用 1组和PF4 +BMP 2m联用 2组 6组 .除正常对照组外 ,余 30只用 6 .5Gy60 Co γ 射线照射 .照射后 10d对动物的骨髓有核细胞数、脾结节、粒 巨噬细胞集落形成单位 (CFU GM)集落数和细胞周期进行检测 .结果 :PF4组小鼠的骨髓有核细胞数显著高于BMP 2m组 (P<0 .0 5 )、照射对照组和PF4 +BMP 2m联用的两组 (P <0 .0 1) .PF4组和BMP 2m组的脾结节数多于照射对照组 (P <0 .0 1) .PF4 +BMP 2m联用 1组除了与PF4 +BMP 2m联用 2组的CFU GM没有明显区别外 ,与其他各组相比均有非常显著的差别(P <0 .0 1) .PF4 +BMP 2m联用 2组的S期的DNA含量最高 .结论 :各组对60 Co γ 射线照射小鼠的造血损伤都具有显著保护和治疗作用 .但PF4和BMP 2m组的作用更好 ,未见明显协同治疗作用 ;联用 2组各项数据除CFU GM集落数外 ,明显比联用 1组增高 ,如将联用组用药间隔的时间再调整 ,可能PF4+BMP 2m联用对造血细胞损伤的保护和促进修复增殖更有利 .

AIM: To compare the effect of PF4, BMP-2m and PF4+BMP-2m on hematopoietic damage in mice exposed to high dose of 60 Co- γ ray and to study the synergism of PF4 and BMP-2m, if any. METHODS: Thirty-six BALB/c male mice were divided randomly into 6 groups: Normal control, irradiated control, PF4, BMP-2m, PF4+BMP-2m 1 and PF4+BMP-2m 2 groups. All the mice except those in normal control group were exposed to 6.5 Gy with 60 Co- γ ray. The hematopoietic parameters of the mice, including the bone borrow cell number, the spleen colony count and the CFU-GM count, were determined on day 10 after exposure to γ ray irradiation. RESULTS: The number of bone marrow cells in PF4 group was remarkably higher than that in BMP-2m group (P< 0.05)and other groups (P< 0.01). The spleen colony count in PF4 and BMP-2m groups were significantly higher than that in irradiated control (P<0.01). The CFU-GM count in PF4+BMP-2m 1 group was higher than that in other groups(P<0.01)except PF4+BMP-2m 2 group. The DNA concentration in PF4+BMP-2m 2 group was the highest. CONCLUSION: Our study suggests that both PF4 and BMP-2m exert protective effects on hematopoietic damage following γ ray irradiation, but PF4 and BMP-2m have no apparent synergism. PF4+BMP-2m 2 group have better effects than PF4+BMP-2m 1 group.