肝细胞肝癌组织中p34^(cdc2)表达的临床意义

Clinical significance of p34^(cdc2) expression in hepatocellular carcinoma tissue

目的 :探讨p34cdc2 激酶在肝细胞肝癌 (HCC)发生发展中的作用及与临床病理特征的关系 .方法 :收集肝细胞肝癌 (含癌旁组织 )标本 4 3例 ,正常肝组织 10例 ,采用免疫组织化学SP法显示p34cdc2 激酶的表达并结合肝癌的临床病理特征进行分析 .结果 :在肝癌组织、癌旁肝组织及正常肝组织细胞中p34cdc2 表达定位于胞质和胞核中 ,在癌组织中过表达 ,差异有统计学意义 (P <0 .0 1) .临床分期I +II期组p34cdc2 阳性表达率高于III+IV期组 (P =0 .0 2 6 ) ;Child Pugh分级A +B级组p34cdc2 阳性表达率高于C级组 (P =0 .0 0 4 ) .而不同病理分级 ,肿瘤大小 ,有无淋巴结转移与p34cdc2 阳性表达率均无统计学意义 (P >0 .0 5 ) .结论 :p34cdc2 激酶的高表达与HCC的发生发展密切相关 ,高表达p34cdc2 激酶可作为反映肝癌临床分期及Child Pugh分级的指标 .

AIM: To study the expression of p34 cdc2 in hepatocellular carcinoma (HCC) and the relationship between the expression of p34 cdc2 and the clinicopathological features of hepatocellular carcinoma. METHODS: Forty-three specimens of hepatocelluar carcinoma tissues (including pericancerous tissues) and ten specimens of healthy hepatocellular tissues were selected. These samples were detected by S-P immunohistochemical method and the clinicopathological features of hepatocellular carcinoma were analyzed. RESULTS: The expression of p34 cdc2 was localized in the cytoplasm and nuclear of cells of HCC tissues and peri-cancerous tissues, brownish yellow and yellow in color. Overexpression of p34 cdc2 was observed in HCC tissues, compared with that in normal liver tissues (P<0.05). The positive expression rate of p34 cdc2 in clinical I+II stages was higher than that in III+IV stages (80.0% vs 42.8%, P= 0.026) , and it was also higher in Child-Pugh grade A+B than in grade C (37.8% vs 0.00 %, P=0.004). But there was no statistical association between various pathologic degrees, tumor sizes, lymphatic metastasis and the positive expression rate of p34 cdc2 (P>0.05). CONCLUSION: There is an overexpression of p34 cdc2 in HCC, which can be used as a marker for clinical stage and the Child-Pugh grade of HCC.