三氯乙烯诱发豚鼠过敏性皮炎的免疫学效应

The immunological effects in guinea pig sensitized by trichloroethylene

目的研究三氯乙烯(TCE)诱发豚鼠过敏性皮炎的免疫学效应。方法采用豚鼠最大值法建立TCE致敏动物模型,应用ELISA双抗体夹心法定量检测血清中IgG含量;用MTT法测定刀豆蛋白A刺激下T淋巴细胞的增殖转化功能,用乳酸脱氢酶释放法测定NK细胞功能。结果TCE的致敏率为667%,属强致敏物。TCE致敏豚鼠血清总IgG含量明显高于阴性对照组(P<005)。TCE致敏豚鼠脾T淋巴细胞增殖转化功能及NK细胞对瘤细胞的杀伤活性与对照组相比无显著性差异(P>005)。结论体液免疫在TCE诱发过敏性皮炎过程中发挥一定的作用。在本染毒方式和染毒剂量下,未见TCE对豚鼠T淋巴细胞和NK细胞活性的影响。

Objective To investigate the changes of certain immunological indexes in development of Guinea Pig allergic dermatitis induced by trichloroethylene (TCE). Methods The Guinea Pig model of TCE-induced allergic dermatitis was established by Guinea Pig Maximisation Test. IgG levels in serum were measured by sandwich enzyme immunoassay. The splenic T lymphocytes ConA-stimulated proliferation function and natural killer (NK) cell activity were detected by MTT assay and micro-LDH release assay, respectively. Results The sensitization rate of TCE was 66.7%. The IgG level in serum of TCE-sensitized animals was significantly higher than that in normal animals (P<0.05). No obvious differences in Splenic T lymphocytes proliferation index and NK cell activity between TCE-sensitized and normal animals were found(P>0.05). Conclusion Humoral immunity may play an important role in development of allergic dermatitis induced by TCE.