硒蛋白对糖尿病小鼠血糖、Ca^(2+)转运以及NO系统影响的实验研究

Effects of selenoprotein on blood glucose,Ca^(2+) transfer and NO system in diabetic mice

目的研究硒蛋白对糖尿病小鼠血糖、Ca2+转运及NO系统的调控作用。方法体重(203±17)g昆明种雄性小鼠,腹腔注射200mgkgbw,2%的四氧嘧啶造糖尿病(DM)模型。实验分6组正常对照组(Ⅰ)、正常+硒蛋白组(Se100μgkgbw)(Ⅱ)、糖尿病对照组(Ⅲ)、DM+硒蛋白低剂量组(Se100μgkgbw)(Ⅳ)、DM+硒蛋白高剂量组(Se300μgkgbw)(Ⅴ)、DM+亚硒酸钠组(Se100μgkgbw)(Ⅵ)。结果Ⅴ组血糖(204±63)mmolL明显低于Ⅲ组(453±33)mmolL,P<005;肾脏三磷酸腺苷酶(Ca2+ATPase)活性,Ⅴ组090±05明显高于Ⅲ组(035±01)μmol(h·mgprot),P<005;一氧化氮合酶(NOS)活性,Ⅴ组(250±43)Uml明显低于Ⅲ组(352±44)Uml,P<005。结论补硒剂量为Se300μgkgbw的硒蛋白能够显著的降低糖尿病小鼠血糖、提高肾脏Ca2+ATPase活性和降低血浆NOS活性。

Objective To study the controling effect of selenoprotein on blood glucose ,Ca^2+ transfer and NO system in diabetic mice.Methods Male Kunming mice of (20.3±1.7) g body weight were injected 200mg/kg bw、2% alloxan in abdomen to make diabetic model(DM),and were randomly divided into six groups:normal control group (Ⅰ),normal+ selenoprotein group(Se 100μg/kg bw) (Ⅱ), DM control group (Ⅲ),DM+ lower dose selenoprotein group(Se 100μg/kg bw) (Ⅳ), DM+ higher dose selenoprotein group(Se 300μg/kg bw) (Ⅴ),DM+ Na_2SeO_3 group(Se 100μg/kg bw) (Ⅵ).Results The level of blood glucose in Ⅴ group[(20.4±6.3 mmol/L] were significantly lower than Ⅲ group(45.3±3.3) mmol/LP<0.05.The activity of Ca^2+ -ATPase of kidney in Ⅴ group(0.90±0.5μmol/(h·mg prot) is significant(ly higher than Ⅲ group[(0.35±0.1)μmol/(h·mg prot)](P<0.05,and the activity of NOS in Ⅴ group[(25.0±4.3) U/ml]is significantly lower than Ⅲ group[(35.2±4.4) U/ml](P<0.05).Conclusion Selenoprotein that supplemented selenium doseis 300μg Se/kg weight could significantly decrease blood glucose、increase the activities of Ca^2+ -ATPase on kidney and weaken the activities of plasma NOS in diabetic mice.