不同发育阶段豚鼠耳蜗外侧壁中mdr_1mRNA的表达及其意义

Expression and Significance of mdr_1mRNA in Guinea Pig Cochlea Lateral Wall at Different Developmental Stages

目的 检测多药耐药基因 1(multidrugresistance 1,mdr1)在不同发育阶段豚鼠耳蜗外侧壁细胞中的表达情况 ,并讨论其在血迷路屏障防护功能中的作用。方法 选取健康白色红目豚鼠 30只 ,其中生后 1～ 2周、3周、4周龄豚鼠各 10只 ,利用原位杂交技术分别检测其耳蜗外侧壁中mdr1mRNA的表达 ,采用MPLAS - 5 0 0计算机图像处理系统进行图像分析。结果 豚鼠耳蜗外侧壁中mdr1mRNA主要在微血管内皮细胞中表达 ;不同发育阶段豚鼠耳蜗外侧壁中的表达强度存在差异 :3周龄与 4周龄豚鼠mdr1mRNA表达强度无显著性差异 (P >0 .0 5 ) ,但两者较 1～ 2周龄豚鼠mdr1mRNA表达强 ,并有显著性差异 (P <0 .0 1)。结论 mdr1mRNA存在于正常耳蜗外侧壁中 ,随着耳蜗外侧壁的发育其表达强度不同 ,可能是不同发育阶段血迷路屏障防护功能不同的机制之一。

Objective To examine the expression and the significance of mdr 1 mRNA in guinea pig cochlea lateral wall at different developmental stages.Methods Thirty white guinea pigs with red eye including three developmental stages were used in this study. The expression of mdr 1mRNA in the cochlear lateral wall was measured in situ hybridization and analyzed by the softeware of MPLAS-500.Results mdr 1mRNA was detected mainly in the endothelial cells.The expression intensity of mdr 1mRNA was different in guinea pig cochlea lateral wall at different developmental stages, mdr 1mRNA in 1～2 weeks group was significantly lower than that of 3-week-group and 4-week-group(P<0.01), there appeared no significant difference between 3-week-group and 4-week-group(P>0.05).Conclusion mdr 1mRNA is found in the normal cochlea lateral wall cells. The expression intensity is different with the development of guinea pig cochlea lateral wall.