脑梗死患者周围血淋巴细胞PPARγmRNA动态变化

The expression of PPARγ mRNA in peripheric lymphocytes in patients with acute cerebral infarct

目的探索脑梗死患者周围血淋巴细胞PPARγmRNA表达变化。方法收集2002年7月～2003年5月在湘雅医院神经内科就诊的发病24h以内的符合试验入选标准的脑梗死患者18例,同时收集相匹配的同期体检者30例做对照。用RT-PCR测定脑梗死组发病第1天、第3天和第6天及正常对照组空腹周围血淋巴细胞PPARγmRNA表达情况。结果脑梗死患者发病第1天、第3天和第6天PPARγmRNA表达值分别为0.233±0.018、0.516±0.027、0.779±0.037,正常对照组PPARγmRNA表达值为0.841±0.065,上述4值两两比较差异显著。结论脑梗死患者周围血淋巴细胞PPARγmRNA表达降低,最低值在发病后第1天,而后逐渐增加,但至发病第6天仍低于正常对照组。

Objective To study the changes of PPARγ mRNA expression in peripheric lymphocyte in patients with acute cerebral infarct.Methods 18 patients at Xiangya hospital experienced cerebral infarcts occurred within 24 hours were consecutively incorporated into the study between July 2002 and May 2003.And 30 healthy subjects were selected as control subjects.The expression of PPARγ mRNA was measured by RT-PCR.Results The levels of PPARγ mRNA expression in patients at first day,third day and sixth day after onset were 0.233±~0.018 ,0.516±0.027,0.779±0.037.And it was 0.841±0.065 in control subjects.There is significance in the above numerical value.Conclusions The expression of PPARγ mRNA decreases in patients with acute cerebral infarct,and might reach the lowest point on 1 day after onset.It is still lower on 6th day compared with the control group.