摘要
采用单一碳源回收菌群的方法与ERIC PCR方法相结合 ,检测水稻 (OryzasativaL )根际施用转基因微生物E4(EnterobacteriacloacaeE4)后 ,其根际微生物的群落结构和多样性的变化 ,进而推测转基因微生物E4在田间施用的安全性。结果表明 :转基因微生物E4施用到水稻根际后 ,水稻根际的代谢指纹图谱和DNA指纹图谱都发生了改变 ,采用southernblot ting检测显示 :E4成为根际的优势菌 ,这对植物生长有利 。
Single carbon source recovery of bacteria and ERIC-PCR techniques were employed in this experiment, to detect the fluctuations of microbial community and diversity in rhizosphere of rice plant after application of transformed Enterobacterera cloacae strain E4 into plant rhizosphere, and to evaluate the safety of releasing strain E4 into fields. Results demonstrated that metabolic fingerprint and DNA fingerprint of microbial community in rhizosphere changed after application of strain E4. Southern blotting profile revealed that E4 turned to be the dominant microbial species in rhizosphere, which is assumed to be beneficial to plant growth without any adverse effect.
出处
《微生物学通报》
CAS
CSCD
北大核心
2004年第6期1-5,共5页
Microbiology China
基金
广东省科技攻关项目 (No 2 0 0 3C2 0 5 1 1 )
关键词
HrpN
根际细菌
群落结构
HrpN, Bacteria in rhizosphere, Community structure