朱砂叶螨体内感染的Wolbachia的wsp基因序列测定与分析

Sequencing and sequence analysis of the wsp gene of Wolbachia in Tetranychus cinnabarinus (Acari: Tetranychidae)

应用Wolbachia的wsp基因特异引物 ,通过PCR扩增法对我国朱砂叶螨Tetranychuscinnabarinus7个地理种群进行了检测。在采自黑龙江佳木斯、安徽安庆、江苏镇江和浙江慈溪的 4个地理种群中扩增出了 5 96bp左右的Wolbachia的wsp基因片段 ,而在河北威县、山东滨州和湖北赤壁 3个地理种群中未发现这个Wolbachia特征基因片段 ,表明Wolbachia在我国朱砂叶螨中的侵染较为普遍。通过对我国朱砂叶螨体内感染的Wolbachia的wsp基因序列进行系统发育分析 ,得出它们全部与B大组的Ori组的Wolbachia株十分相近或完全相同 。

Specific primers derived from the wsp gene sequence were used to amplify DNA of Wolbachia from seven populations of Tetranychus cinnabarinus in China by polymerase chain reaction (PCR). The wsp characteristic 596 bp products were observed after amplification of extracts from four geographical populations (Jiamusi population of Heilongjiang Province, Anqing population of Anhui Province, Zhenjiang population of Jiangsu Province and Cixi population of Zhejiang Province) of T. cinnabarinus, but in other three populations (Weixian population of Hebei Province, Binzhou population of Shandong Province and Chibi population of Hubei Province) this gene was not found. The results showed that the infection of Wolbachia is widespread in T. cinnabarinus in China. By analyzing the wsp gene sequences of Wolbachia in T. cinnabarinus through constituting the phylogenetical tree, we found that they were similar to or completely identical with the wsp gene sequence of some strains of Ori group in B supergroup of Wolbachia, and this indicates that they may be the same strain of Wolbachia or at least they are closed-related sister strains.