摘要
目的 :研究银杏内酯 B对大鼠脑缺血再灌注神经元损伤的干预作用及其可能机制。方法 :采用Kam eyam a的 3条动脉夹闭法制备大鼠脑缺血再灌注损伤模型。 4 5只 Wistar大鼠随机分成假手术对照组、缺血再灌注模型组、生理盐水对照组和银杏内酯 B预处理组 ;测定各组动物脑匀浆液中超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH Px)和 ATP酶活性以及丙二醛 (MDA)含量 ,并观察脑组织的病理学改变和细胞凋亡指数。结果 :全脑缺血再灌注可引起 SOD、GSH Px和 ATP酶活性明显下降 ,MDA含量显著升高 ;脑组织呈现炎症性改变 ,层次不清 ,细胞凋亡指数达 (40 .2± 6 .3) %。银杏内酯 B(1～ 10 mg/ kg)能剂量依赖性地降低 MDA水平 ,提高 SOD、GSH Px和 ATP酶活性 (P<0 .0 5或 P<0 .0 1) ,明显减轻脑组织神经细胞损害 ,减少细胞凋亡发生率。结论 :银杏内酯 B对大鼠脑缺血再灌注神经元损伤具有明显的保护作用 ,其机制可能是通过减少自由基的产生和增强 ATP酶活性。
Objective: To investigate the protective effects and mechanism of ginkgolide B(银杏内酯B, GB) on neuron injury induced by cerebral ischemia/reperfusion(I/R) in rats. Methods: The bilateral cerebral hemisphere I/R model was established by ligating three arteries with Kameyama's manner. Fortyfive Wistar rats were randomly assigned to sham operation control, I/R model, normal saline control (NS) and GB pretreatment group. The activity of superoxide dismutase(SOD), glutathione peroxidase(GSHPx), ATPase , and the contents of malondialdehyde(MDA) in brain tissue were measured. Apoptosis of neurons and pathologic changes were observed. Results: Compared with sham operation control group,the values of SOD, GSHPx and ATPase were reduced, while MDA level increased significantly in I/R group whose neuron apoptosis index was (40 2±6 3)%. The inflammatory changes were found in brain tissue and rant order of cells wasn't clear. Previous administration of GB (110 mg/kg) could reduce MDA level and increase SOD, GSHPx and ATPase activities dosedependently( P <0 05 or P <0 01), could remarkably alleviate the brain tissue injury induced by I/R, and reduce the apoptosis index to (24 3±5 1)%, (21 0±4 2)% and (9 4±3 3)% respectively with different dosage of GB( P <0 05 or P <0 01). Conclusion: GB possesses a markedly protective effects on I/Rinduced cerebral neuron injury by reducing the production of free radicals , enhancing the free radical scavenger system and ATPase activity.
出处
《中国中西医结合急救杂志》
CAS
2005年第1期17-20,共4页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
安徽省教育厅自然科学基金资助项目 (2 0 0 3 kj3 0 2 )
