幽门螺杆菌感染Beagle犬动物模型研究

Study on the Beagle dog model infected with Helicobacter pylori

目的 建立幽门螺杆菌感染Beagle犬动物模型 ,为研究幽门螺杆菌 (Helicobacterpylori,Hp)感染的临床治疗及预防提供实验基础。方法 对Beagle犬口服灌喂Hp液体培养悬液 ,通过胃镜观察其胃部组织病理改变 ,酶试验、组织培养、粪便PCR、血清及唾液中抗体和RT PCR法检测脾脏中淋巴细胞细胞因子含量等方法对Hp感染Beagle犬情况进行分析。 结果 Beagle犬感染Hp数月后胃部组织仍有病理改变 ;组织培养、酶试验、粪便PCR及抗体、细胞因子检测均阳性。 结论 Hp可以长时间定植于Beagle犬体内并引起相应的病理改变 ,其可以作为Hp感染动物模型进行相关实验研究。

A Beagle dog model was established for the studies on the clinical treatment and prevention for the infection with Helicobacter pylori(Hp). The Beagle dogs were orally inoculated with broth cultures of Hp, and the pathological changes on the gastric tissues were observed through gastroscopy examination. In addition, enzyme testing, tissue culture, PCR assay of fecal samples, antibody determinations in sera and saliva as well as the contents of cytokines produced by lymophocytes of spleen as demonstrated by RT PCR were also performed in this study.It was found that, after several months of infection with Hp, the histopathological changes were still observed in the gastric tissues of the Beagle dogs infected,all the other testings mentioned above showed positive responses.It is concluded that Hp can localize on the mucosa of the Beagle dogs for a long time and cause definite pathological changes, and the Beagle dog model can be used as a better animal model for the studies ofHp.