一种高灵敏度测定血红蛋白的荧光分析法

Highly Sensitive Spectrofluorimetric Determination of Hemoglobin Based on Hemoglobin Enzymatic Activity to Catalyze the Oxidation of bis (Salicylaldehyde)-o-Phenylenediimine

建立了以双水杨醛邻苯二亚胺为酶催化氧化底物,血红蛋白(Hb)作为过氧化物模拟酶,在Tween 80介质中的酶催化反应测定血红蛋白的方法,并探讨了反应机理.讨论了利用本体系测定Hb的最佳条件,结果表明:在优化的条件下,Hb浓度在5.0×10-10～5.0×10-8 mol/L范围内呈良好的线性关系,方法的检出限为2.6×10-11 mol/L.本方法与其它测定Hb方法相比具有更高的灵敏度.

A highly sensitive catalytic-fluorimetric method based on hemoglobin ( Hb) enzymatic activity to catalyze the oxidation of bis ( salicylaldehyde)-o-phenylenediimine in the presence of Tween-80 has been developed. Reaction mechanism was discussed. The optimal experimental conditions for the determinations were established. The linear range of the method was 5.0 x 10(-10) similar to 5.0 X 10(-8) mol/L of Hb. The detection limit was calculated to be 2.6 x 10(-11) mol/L. The proposed method allows for the determination of Hb with grater sensitivity than other reported ones.