大肠埃希氏菌和肺炎克雷伯氏菌的药敏试验

Drug Sensitivity Test of the E.Coli and the KL.Pneumoniae

大肠埃希氏菌和肺炎克雷伯氏菌是动物肠道内的常见细菌,体弱和幼龄动物受气温变化或食物变换等因素的影响时,肠道内的这些条件性病原菌便大量繁殖,引起胃肠道疾病或败血病。采集这2种病原菌EL-1株(大肠埃希氏菌)和EL-2株(肺炎克雷伯氏菌),在培养基上培养生长,再制备各种抗菌药片进行药敏试验。经动物试验肠霉素试验和抗原性研究,证明EL-1株和EL-2株能致死小白鼠,能产生肠毒素,具有较好的抗原性和较强的毒性。本试验通过药敏试验来检测这2种病原菌对不同抗菌药物的敏感性,以选择出有效药物用于预防和治疗这类疾病。试验表明,EL-1株和EL-2株除对氟哌酸、氟嗪酸、氯霉素和复方磺胺抗菌素有较高敏感性外,对其它抗菌药物具有不同程度抗药性。

are common bacterium in the animal intestinal tract. When the animals with physically weak or young age affected by the factors of temperature change or food transformation, these condition disease germs in the intestinal tract have massively reproduce. This cause the disease or the defeat hemopathy of the stomach and intestine mucosa. Gathers these two kinds of disease germs: Strain EL-1 (E.Coli) and Strain EL-2 (KL.Pneumoniae), raised on the culture medium, then prepares each kind of antibacterial pill enters the drug sensitivity test. After the animal experiment intestines mildew element experiment and the antigenicity research, they can proves that Strain EL-1 and Strain EL-2 can risk the life of the white mice. And they can be able to produce the intestines toxin. They have a better antigenicity and a stronger toxicity. Through the drug sensitivity test examined these two kinds of disease germs to the different antibiotics sensitivity, we can chooses the effective medicine to prevent and treat this kind of disease. The experiment indicates, Strain EL-1 and Strain EL-2 have the high sensitivity to the Norfloxacin, the Ofloxacin, the Chloramphenico and the duplicate side Sulfa antibiotics, but they have the different antibiotic nature to other antibiotics.