食物脂肪对大鼠脑、肝、肾组织中脂质过氧化作用的影响

Effects of Dietary Fat on Lipid Peroxidation Level in Rats

目的探讨高不饱和脂肪酸食物对实验动物脑、肝、肾组织中脂质过氧化作用的影响及其组织特异性。方法采用美国国家毒理研究中心(NCTR)繁殖的雌性F344大鼠75只,用含3%,5%,10%,15%和20%玉米油的饲料分别喂养2、10和20周。定量分析不同剂量组及喂养时间F344大鼠脑、肝、肾组织中丙二醛(MDA)的浓度。结果用含3%和5%玉米油饲料喂养的大鼠组织中MDA含量明显高于高脂肪喂养组(P<0.05),而20周喂养组大鼠组织中MDA含量明显低于2周和(或)10周喂养组(P<0.05)。脑组织中MDA含量明显高于肝、肾组织(P<0.05)。脑组织在喂养2周时,肝、肾组织在喂养10周时MDA含量最高。结论不同组织中MDA变化在时间窗口中也显示出组织特异性,脑组织似乎更容易受到脂质过氧化作用的损害。食物脂肪对体内脂质过氧化作用的影响具有组织特异性。

Objective To explore the effects of dietary fat on lipid peroxidation in female F344 rats. Methods The rats were fed diets containing various levels of corn oil ( 3%, 5%, 10%, 15%, or 20% ) for 2, 10, 20 weeks and to detect the malondialdehyde (MDA) concentrations in cerebellum, kidney and liver tissues by HPLC method. Results It was observed that rats consuming 3% and 5% corn oil diets yielded significantly higher levels of MDA compared with those fed with higher fat diets. It was also observed that the MDA levels at group of 20 weeks of feeding were significantly lower than groups of 2 weeks and/or 10 weeks of feeding. The three organs studied showed different MDA levels. MDA level in cerebellum was obviously higher than that in liver and kidney. Conclusion The lipid peroxidation linking with dietary fat seems to have tissue specific and time specific. The cerebellum tissues seem easy to be attacked by lipid peroxidation linking with dietary fat. The peaks of MDA concentrations emerge were at 2 week time point in cerebellum and at 10 week time point in liver and kidney respectively.