表面活性剂引起的血红蛋白构象变化

Conformational Changes of Hemoglobin Induced by Surfactants

目的通过研究表面活性剂对血红蛋白构象变化的影响,探讨环境中存在的表面活性剂对人类健康的隐患。方法利用同步荧光光谱法、紫外可见吸收光谱法、电化学分析法和原子力显微镜,对阴、阳离子表面活性剂作用下血红蛋白的光谱性质、电化学行为及直观形貌拓扑图进行表征。结果采用同步荧光光谱、紫外吸收光谱、电化学行为和原子力显微镜都可以观察到阴离子表面活性剂十二烷基硫酸钠对血红蛋白的构象变化影响明显。结论阴离子表面活性剂十二烷基硫酸钠可以引起血红蛋白的构象改变。

Objective To study the effects of surfactants on conformation of hemoglobin and further to understand the potential impact of surfactants in environment on human health. Methods The changes of the conformation of hemoglobin induced by cation and anion surfactants were investigated with four methods, such as synchronous fluorescence spectra, UV, electrochemistry and atom force microscope. Results The changes of the conformation of hemoglobin induced by anion surfactant were obvious but were not by cation surfactant. Conclusion Sodium lauryl sulphate, an anion surfactant, may change the conformation of hemoglobin.