四氯化碳/乙醇诱导129/Sv小鼠产生原发性肝细胞癌模型的建立

Establishment of the primary hepatocellular carcinoma model of 129/Sv mice induced by Carbon Tetrachloride (CCl 4)/ethanol

目的 建立稳定可靠的肝癌动物模型 ,为深入进行肝细胞癌的实验研究打下良好的基础。方法 取 90只 12 9/Sv小鼠 ,采用四氯化碳 /乙醇诱导 2 0周 ,观察其肝癌发生的情况。另取 10只作为正常对照组。结果 90只 12 9/Sv小鼠共死亡 4 7只 ,最后剩余的 4 3只小鼠中有 16只发生肝癌。而常对照组 10只小鼠 ,其肝脏均未发现明显异常。诱导组小鼠的肝细胞癌类型以中、高分化为主。结论 采用四氯化碳 /乙醇诱发的 12 9/Sv小鼠肝癌 ,模型稳定 ,癌变率高 ,经历了肝炎、肝硬化阶段 ,与人肝癌的发生发展过程非常相似。因此 ,是较理想的研究肝癌的实验动物模型。

Objective To establish a stable and credible animal model of HCC. Methods Ninety 129/Sv mice were induced for twenty weeks by CCl 4/ethanol,and then the occurrence and development of hepatocellular carcinoma (HCC) were observed. Another ten 129/Sv mice were used as normal control. Results Of all the ninety 129/Sv mice induced by Carbon Tetrachloride (CCl 4)/ethanol, only forty three were left alive with hepatocellular carcinogenesis in sixteen mice. There was no abnormality of liver in the 10 normal mice. Most of the induced hepatocellular carcinoma were of middly to highly differentiated. Conclusions The HCC model of 129/Sv induced by CCl 4/ethanol is stable and the rate of carcinogenisis is high. The course of hepatitis, liver fibrosis and cirrhosis is very similar to that of human HCC. So this model is an ideal and suitable one for us to study the occurrence and development of human HCC.