TIMP-2对人脑胶质瘤U87体外侵袭力的抑制作用

Effects of recombinant adenovirus AdTIMP-2 on the invasiveness of human U87 glioma cells in vitro

目的 探讨TIMP2对人脑胶质瘤细胞体外侵袭力的抑制作用。方法 用携带TIMP2基因的重组腺病毒载体AdTIMP2,体外转染人胶质瘤细胞系U87,用Westernblotting检测目的蛋白的表达。通过Boyden小室法检测U87转染前后细胞体外侵袭力的变化,同时采用胶原酶谱法分析MMP2、MMP9活性改变。结果 D转染AdTIMP2病毒后的U87细胞目的蛋白表达上调,转染后U87细胞的MMP2、MMP9明显下降,同时体外侵袭力受到明显抑制。结论 重组腺病毒载体介导的TIMP2基因治疗,是一种有效的抗胶质瘤方法。

Objective We evaluated the suppressive effect of metalloproteinases-2 (TIMP-2) gene. on the invasiveness of human U87 glioma cells.Methods After transfection of U87 human glioma cells with the recombinant adenovirus in vitro, the expression of TIMP-2 was confirmed by Western blotting. We measured the invasiveness of U87 cells by Boyden chamber assay and the relative activity of MMP-2、MMP-9 by gelatin zymography. Results TIMP-2 was expressed stably in glioma cells. The activity of MMP-2、MMP-9 and invasiveness of human U87 glioma cells were inhibited when transfected with AdTIMP-2. Conclusion Our studies suggested that adenovirus-mediated TIMP-2 gene therapy might be useful for treatment of malignant glioma.