人参皂苷Rg1延缓细胞衰老过程中端粒长度和端粒酶活性的变化

Roles of telomere and telomerase in the process of ginseno- side Rg1 protection against tert-butyl hydroperoxide-induced senescence in WI-38 cells

目的 观察人参皂苷Rg1延缓细胞衰老作用过程中细胞端粒长度以及端粒酶活性的变化。方法 采用三丁基过氧化氢(tert butylhydroperoxide,t BHP)体外诱导WI 38细胞衰老,从超微结构、细胞周期分析及SA β 半乳糖苷酶 (Se nescenceassociatedβ galactosidase)化学染色等指标观察细胞衰老,Southernblotting结合PCR-ELISA方法检测端粒长度和端粒酶活性。结果 t BHP可成功诱导建立体外细胞衰老模型,出现衰老细胞的特征性改变,细胞端粒长度明显缩短。Rg1可明显减弱t BHP诱导细胞衰老的作用,减少t BHP引起的端粒长度缩短,同时可见细胞端粒酶活性的表达。结论 人参皂苷Rg1可能通过激活端粒酶活性和减少端粒长度缩短而发挥其抗t BHP诱导的WI 38细胞衰老作用。

Aim To investigate the roles of telomere and telom er ase in the process of ginsenoside Rg1 protection against tert-butylhydroperoxid e (t-BHP)-induced senescence in WI-38 cells.Methods Senile W I-38 cells were induced by t-BHP in vitro. Cell ultrastructure, cell cycle assay and β-galactosidase cytochemistry staining were used to evaluate cell s enescence. Terminal restriction fragment (RTF) length and telomerase activity we re detected by southern blotting and PCR-ELISA, respectively.Results t-BHP induced cell senescence with characteristics of shortenned RTF len gth. Pretreatment with Rg1 significantlyattenuated t-BHP-induced senescence in WI-38 cells. Compared with c ells treated with t-BHP alone, Rg1 pretreatment markedly decreased the shorteni ng of RTF length and resulted in telomerase activation.Conclusion Activation of telomerase and prolonging of RTF length might be involved in t he process of ginsenoside Rg1 protection against t-BHP-induced senescence in W I-38 cells.