一氧化氮在实验大鼠骨折愈合中的作用研究(英文)

Function of nitric oxide in fracture healing in experimental rats

目的探讨一氧化氮在骨折愈合中的可能作用。方法Wistar大鼠120只随机分为三组:L-精氨酸组,L-硝基-精氨酸甲基脂(L-NAME)组及对照组,建立股骨闭合性骨折模型,骨折后3 d,1,2和4周分别取每组10只大鼠骨折处骨痂行大体及HE染色观察。结果大体所见,在骨折后不同时期L-NAME组骨折处骨痂生成少,骨痂塑形慢,L-精氨酸组与其正好相反。L-NAME有使骨折愈合延迟的趋势,但与对照组比较并没有显著的意义(P =0.10);L-精氨酸组大鼠无论在骨痂塑形和骨折愈合程度上都强于L-NAME组(P <0.05)。结论在骨折愈合过程中加入L-NAME使骨折愈合减慢,而加入L-精氨酸会促进骨折愈合。一氧化氮可能促进骨折的愈合过程。

To evaluate the possible function of NO in fracture healing. 120 Wistar rats were divided into 3 groups including L-arginine, L-NAME and the control group. The closed fractured model of each rat was built in the right femur. The callus of the fractured site was amputated and observed by the gross appearance and HE stain in the 3rd day, the first, second and fourth week after fracture. The gross observation of fracture in different stage indicated relatively small callus after the L-NAME intake. This was the same with the callus modelliing. Compared to these findings, completely opposite results were seen in the L-arginine group. When compared to the control group, the tendency of fracture healing was seen in the L-NAME group, but it had no significant difference (P =0.1). The degree of remodeling of the bone and fracture healing in the group of L-arginine was stronger than that in L-NAME (P <0.05). [Conclusion] L-NAME's intake will inhibit the process after the fracture, which indicates NO may play an important role in the process of fracture healing.