缺血预处理对大鼠肝缺血再灌注损伤后早期细胞CyclinD_1mRNA表达的影响

Effect of ischemic preconditioning on CyclinD_1 mRNA expression of liver cell during early ischemic reperfusion in rats

目的观察缺血预处理对大鼠肝缺血再灌注损伤早期细胞CyclinD1 mRNA表达变化的影响。方法采用大鼠原位部分缺血再灌注模型,54只SD大鼠随机分为缺血再灌注组(IR),缺血预处理组(IP)与假手术组(SO组),应用RT-PCR法检测各组复灌后0,1,2,4 h肝组织CyclinD1 mRNA的变化。结果与IR组相比,IP组在复灌早期(0,1 h),肝组织的CyclinD1 mRNA表达明显增高。(0.568±0.112 vs 0.274±0.069,0.762±0.164 vs 0.348±0.093,P<0.01)。结论缺血预处理可促进肝细胞在缺血再灌注损伤后早期Cy-clinD mRNA的表达。

To observe the effect of ischemic preconditioning on the cyclinD1 mRNA expression of rats liver cell during early ischemic reperfusion. Fifty-four SD rats were randomly divided into ischemic preconditioning group (IP), ischemia/Reperfusion group (IR) and sham operation group (SO). The model of partial liver ischemia/reperfusion was used. The specimen was collected at 0, 1, 2, 4 h after ischemia/reperfusion whether there was ischemic preconditioning or not. In each group, the cyclinD1 mRNA was detected by RT-PCR. Compared with IR group, the cyclinD1 mRNA expression was significantly different at early reperfusion (0, 1 h), in IP group. (0.568±0.112 vs 0.274±0.069, 0.762±0.164 vs 0.348±0.093, P<0.01). [Conclusion] Ischemie preconditioning before hepatic ischemia/reperfusion can promote the cyclinD1 mRNA expression in the early reperfusion.