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缺血再灌注后胆管上皮细胞凋亡和bcl-2/bax的表达及意义 被引量：2

Apoptosis and bcl-2/bax expression induced by warm ischemia-reperfusion in rat bileduct epithelial cell
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摘要 目的探讨肝脏经不同时间热缺血再灌注后胆管上皮细胞的凋亡和bcl-2/bax的表达及意义。方法以大鼠肝脏原位缺血再灌注为模型,经15、30和60 min缺血再灌注6 h后取材,用TUNEL法原位检测胆管上皮细胞的凋亡,免疫组织化学方法检测bcl-2/bax在胆管上皮细胞中的表达。结果随着缺血时间延长,经再灌注后发生凋亡的胆管上皮细胞逐渐增多,bcl-2/bax蛋白表达指数呈进行性降低,bax则随凋亡细胞的增多表达增强。结论热缺血再灌注损伤引起胆管上皮细胞的凋亡,并随缺血时间的延长而加重,bcl-2/bax凋亡基因介导了缺血再灌注后损伤引起的胆管上皮细胞的凋亡,bcl-2对细胞凋亡具有抑制作用。 To elucidate the dynamic changes of apoptosis and bcl-2/bax in rat bileduct after warm ischemia-reperfusion injury. TUNEL technique and immunohistochemistochemisty were carried out to determine apoptotic cells in situ and the expression of bcl-2/bax preteins tespectively in 24 bileduct specimens of rat liver ischemia reperfusion model. With the prologation of warm ischemia time, apoptotic bileduct epithelial cells increased, and the expression of bcl-2 dropped greatly. [Conclusions] Ischemia-reperfusion injury induces bileduct cell apoptosis with the regtulation of bcl-2/bax protions.
作者 徐文辉 蔡方刚 叶启发 夏穗生
机构地区 华中科技大学同济医学院附属同济医院 中南大学湘雅三医院湘雅移植医学研究院
出处 《中国现代医学杂志》 CAS CSCD 北大核心 2005年第1期61-63,共3页 China Journal of Modern Medicine
关键词 缺血再灌注损伤 胆管上皮细胞 BCL-2/BAX ischemia-reperfusion injury bileduct epithelial bcl-2/bax
分类号 Q251 [生物学—细胞生物学]
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二级参考文献16

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共引文献13

同被引文献22

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中国现代医学杂志
2005年 第1期

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