摘要
用5份在历年大麦花药离体培养中反应值低的基因型作供试材料,比较了早、晚播,培养主茎穗或分蘖穗中花药和诱导培养基中加高、低浓度生长素的花培反应值,结果表明:早播,用主茎穗花药和诱导培养基中加高浓度生长素,可以提高迟钝基因型的反应花药率和绿苗率。
Early or late-sowing,culturing anthers collected from spikes of main stem or tiller and inducing media with lower or higher concentration of auxins were compared for their effects on anther culture responsiveness using 5 barley genotypes which previously proved to be insensate to anther culture.According to the comparison,frequencies of anther responding and green plant of the insensate genotypes were able to be improved by early- sowing,culturing anthers of main-stem spikes and media with higher concentrations of aux- ins.
出处
《上海农业学报》
CSCD
1993年第4期19-22,共4页
Acta Agriculturae Shanghai
基金
中华人民共和国农业部"八五"生物技术研究重点资助项目
关键词
大麦
花药培养
迟钝基因型
绿苗率
Barley
Anther culture
Insensate genotype
Freq.of anther responding
Freq.of green plantlet
