摘要
目的建立参茸胶囊中人参皂苷Rg1、人参皂苷Re的含量测定方法。方法采用高效液相色谱法 ,色谱柱为Kromasil5 μmC1 8柱 ( 4 .6mm× 2 0 0mm ,5 μm) ,流动相为 0 .0 5 %磷酸—乙腈 ( 80 :2 0 ) ,流速为1 .0ml.min- 1 ,检测波长为 2 0 3nm。结果人参皂苷Rg1进样量在 0 .990~ 6.60 0 μg的范围内与峰面积呈良好的线性关系 ,相关系数为 0 .9998;人参皂苷Re进样量在 0 .81 6~ 5 .440 μg的范围内与峰面积也呈良好的线性关系 ,相关系数为 0 .9998。平均回收率分别为Rg1 =98.5 1 %、Re =98.5 0 % ;RSDRg1 =0 .82 % ,RSDRe=1 .0 7%。结论本方法简便快速 ,分离度好 。
Objective To determine Ginsenoside in Shenrong Capsule by HPLC.Methods Samples were purified by C 18 column.HPLC comumn was Dikma Diamonsil C 18(4.6mm×200mm,5μm),mobile phase was 0.05%phosphate-Acetonitrile(80:20),flow rate was 1.0 ml.min -1 and detection wavelength was 203nm.Results The calibration curve was linear in the range of 0.990~6.600μg for Ginsenoside Rg1(r=0.9998);The calibration curve was linear in the range of 0.816~5.440μg for Ginsenoside Re(r=0.9998)too.The average recovery were Rg1=98.51%、Re=98.50%.RSD were 0.82% and 1.07%.Conclusion The method with good separation is conwenient,rapid,accurate and reliable.
出处
《现代中医药》
CAS
2005年第1期61-63,共3页
Modern Chinese Medicine