青藤碱微透析回收率测定方法的体外模拟实验

In - Vitro Experiment for Detecting Microdialysis Recovery rate of Sinomenine

[目的]研究利用体外模拟方式测定青藤碱微透析回收率的方法及影响因素。[方法]回收率的测定采用浓差法(增量法、减量法)及零净通量法(ZNF法)。[结果]增量法及减量法测定的回收率一致;回收率与媒体中青藤碱的浓度无关;浓差法的日内稳定性及重现性均好;利用零净通量法能准确地测定媒体中青藤碱的浓度及其回收率。[结论]微透析取样技术可用于青藤碱的药动学研究,浓差法中的减量法(即体内研究中的反向透析法)可作为体内微透析研究中青藤碱回收率的测定方法。

[Objective] To explore the in-vitro determination method for the recovery rate of sinomenine in microdialysis probe and to investigate the influencing factors. [Methods] The recovery rate of sinomenine was detected by concentration difference method (by gain and by loss) and zero-net flux method. [Results] The recovery rate detected by increment method was as the same as that by decrement method; the recovery rate had no correlation with the sinomenine concentration in the solvent. Sinomenine recovery assessed by concentration difference method had a good intra-day stability and intra-day reproducibility. Sinomenine concentration and recovery in the solvent could be determined accurately by zero-net flux method. [ Conclusion ] Microdialysis sampling can be used for the pharmacokinetic study of sinomenine and decrement method (ie. retrodialysis) can be used for the determination of recovery rate of sinomenine.