用双核微核实验检测多环芳烃的致突变性

The Mutagenicity of Polycyclic Aromatic Hydrocarbon Detected by Bionuclears Micronucleus Assay

探讨了大气中常见的5种多环芳烃的致突变性。采用人外周血淋巴细胞胞质分裂阻滞微核实验检测不同浓度5种多环芳烃对人血淋巴细胞的微核诱导作用。指出5种多环芳烃各染毒剂量组微核率与阴性对照组相比有显著性差异,并且有明显的剂量反应关系,说明他们均可引起人外周血双核淋巴的微核率增加。除苯并(a)芘(BaP)外其余的几种多环芳烃也具有一定的潜在危害,应引起高度重视。

Objective:To detect the mutagenicity of the five types of polycyclic aromatic hydrocarbon (PAHs) in the air. Method : We used cytokinesis-block micronucleus assay to study the micronucleus induction of human blood lymphocytes of different concentrations of five polycyclic aromatic hydrocarbon(Benzopyrene(BaP), Benzoanthracene, CHR,PER,pyrene (PYR)). Results: The micronucleus rates of different concentrations of five types of PAHs were significantly increased compared with control group, P<0.01,and they also have obvious dose-effect relationships. These suggested that they can increase the blood lymphocytes micronucleus rate. Conclusion: Except for BaP other PAHs also have potential hazard to human, and we should pay more attention to them.