恶性肿瘤患者外周血细胞肿瘤转移促进基因的表达检测

Measurement of the expression level of tumor metastasis-enhancing genes in peripheral blood cells of patients with malignant carcinomas

目的:探讨肿瘤转移促进基因在恶性肿瘤患者外周血细胞中的表达规律。方法:采用流式细胞术对173例各种恶性肿瘤患者外周血细胞的CD44S+、CD44V5+、CD44V6+和cerbB2+细胞表达率进行了检测和分析。结果:恶性肿瘤患者外周血细胞CD44V5、CD44V6和cerbB2表达率均显著高于正常对照组,P<001或P<005;在不同肿瘤类型中,不同肿瘤转移促进基因上调的幅度明显不同:胃癌以CD44V5表达率为最高,大肠癌以CD44V6为最高,卵巢癌、宫颈癌和乳腺癌则以cerbB2为最高;肿瘤转移者显著高于未转移者,P<001或P<005。而恶性肿瘤患者外周血细胞CD44S的表达率的变化规律与CD44V5、CD44V6、cerbB2的表达完全相反,它们之间差异均有统计学意义,P<005。但CD44S在各种类型肿瘤患者血细胞中的表达率之间均差异无统计学意义,P>005。结论:恶性肿瘤能使患者外周血细胞的肿瘤转移促进基因表达率出现某些异常;而这种表达异常与恶性肿瘤转移的关系十分密切;不同肿瘤患者血细胞表达的肿瘤转移促进基因的类型有一定的倾向性。

OBJECTIVE:To study the expression regularity of tumor mtastasis- enhancing genes in peripheral blood cells from patients with malignant carcinoma.METHODS: The expression rates of CD 44S+,CD 44V 5+, CD 44V 6+ and c-erbB-2+ cells in peripheral blood of 173 patients with malignant carcinomas were measured and analyzed by flow cytometry.RESULTS: The expression rates of CD 44V 5+,CD 44V 6+ and c-erbB-2+ cells in peripheral blood cells of patients with malignant carcinomas were higher than those of normal control groups, P<0.01 or P<0.05.The expression rate of every tumor metastasis-enhancing genes in peripheral blood cells of patients with different tumor kind was significantly different, P< 0.01 or P<0.05.The expression rate of CD 44V 5+,CD 44V 6+, c-erbB-2+ cells of patients with metastasis were higher than those of patients with non-metastasis, P< 0.01 or P< 0.05.While the expression regularity of CD 44S+ cells in peripheral blood cells for patients with malignant tumor were contrary changes for comparison with CD 44V 5,CD 44V 6,c-erbB-2 and these were significantly different, P<0.05. The expression rate of CD 44S+cells in peripheral blood cells were no significantly different in patients with different kind malignant tumor, P>0.05. CONCLUSIONS: Malignant tumors may induce abnormal expression of tumor metastasis-enhancing gene in peripheral blood cells of patients with malignant tumor, which were related to tumor metastasis. There are some tendency in the expression of tumor metastasis-enhancing gene according to the the tumor kind. Chin J Cancer Prev Treat,2005,12(2):99-102