非小细胞肺癌组织中FAK和pY397FAK的表达

Expressions of focal adhesion kinase and phosphorylated focal adhesion kinase in non-small cell lung cancer

目的:探讨粘着斑激酶(focaladh sionkinase,FAK)和磷酸化FAK(phosph FAK,pY397FAK)在非小细胞肺癌(no smallcelllungcancer,NSCLC)和正常支气管 黏膜上皮中的表达,及FAK在NSCLC发生 浸润和转移中的作用。方法:应用免疫组织 化学方法检测FAK和pY397FAK在60例 NSCLC和19例正常支气管黏膜上皮中的表 达,并与临床病理指标和Ki 67表达进行联 合分析。结果:FAK和pY397FAK在NSCL 中表达为83.3%(50/60)和55.0%(33 60),高于正常支气管黏膜上皮[57.9%(11 19)和21.1%(4/19)],差异有统计学意义 P=0.030、P=0.010。FAK和pY397FAK表 达升高与TNM分期及淋巴结转移有关,在 Ⅲ期和淋巴结转移组的阳性率高于Ⅰ+Ⅱ 期和无淋巴结转移组,差异有统计学意义, 均<0.05。FAK的强阳性表达率在低分化 组中(73.1%,19/26)显著高于高 中分化组 (47.1%,16/34),P=0.043。结论:FAK和 pY397FAK过表达在NSCLC发生和进展中发 挥重要作用。FAK和pY397FAK可能作为判 断NSCLC生物学行为的指标和治疗的新靶 点。

OBJECTIVE: To explore the roles of FAK and its phosphorylated form, i.e.pY397FAK, in tumorigenesis, invasion and metastasis of non-small cell lung cancer (NSCLC) by detecting the expressions of FAK and pY397FAK in human normal bronchiolar epithelium and primary NSCLC.METHODS: By using the immunohistochemical method, the expressions of FAK and pY397FAK in 60 cancerous tissues and 19 normal bronchiolar epithelia were measured. The relationship between FAK, pY397FAK expressions and clinicopathological parameters as well as Ki-67 expression was analyzed.RESULTS:Elevated levels of FAK and pY397FAK expressions were detected in primary tumor (83.3%, 55.0%) compared with normal bronchiolar epithelium (57.9%, 21.1%), P=0.030,P=0.010. The expressions of FAK and pY397FAK were related with TNM stage and the lymph node involvement,P<0.05.The positive rates of FAK and pY397FAK in stage Ⅲ and lymph node metastasis groups were significantly higher than those in stage Ⅰ+Ⅱ and no lymph node metastasis groups, P<0.05. The strongly positive rate of FAK expression was 73.1% in poorly differentiated cases, 47.1% in well-moderately differentiated cases; the difference was significant between the two groups,P=0.043.CONCLUSIONS: FAK and pY397FAK are overexpressed in NSCLC and may play important roles in the pathway of carcinogenesis and progression of NSCLC. FAK and pY397FAK may be used as important indices for biologic behavior of NSCLC and identified as new therapeutic targets.