上皮性卵巢肿瘤组织中端粒酶活性的表达及与临床病理因素相关性研究

Expression of telomerase activity and its relationship with clinical-pathological factors in epithelial ovarian tumors

目的:探讨端粒酶在卵巢癌发 生和发展中的作用,及端粒酶活性与临 床病理因素的相关性。方法:应用端粒 重复序列扩增技术(TRAP)检测45例卵 巢上皮性肿瘤及9例正常卵巢组织端粒 酶活性。结果:端粒酶阳性检出率在恶 性和交界性卵巢肿瘤组中分别为 83.33%(25/30)和5/7,差异无统计学意 义,P=0.5963;良性上皮性卵巢肿瘤及 正常卵巢上皮组织中未检测到端粒酶活 性,与前两组之间均差异有统计学意义 P<0.01。端粒酶的表达与临床病理因 素无明显相关性。结论:端粒酶在卵巢 癌发生和发展过程中可能具有重要作 用,并有望作为卵巢上皮性癌基因治疗 的靶点。

OBJECTIVE:To investigate the role of telomerase in the development of ovarian carcinoma, and to ascertain the co-relationship between telomerase activity and clinical-pathological factors.METHODS:The telomerase repeat amplification protocol (TRAP) assay was used to examine telomerase activity in forty-five ovarian tumors and nine normal ovarian tissues.RESULTS: The positive rates of ovarian carcinoma and borderline ovarian tumors were 83.33%( 25/30) and 5/7 respectively, but none of the benign ovarian tumors and normal ovarian tissues was detected to have telomerase activity, P=0.596 3. The positive rates of telomerase activity in ovarian carcinoma and borderline ovarian tumors were significantly higher than those in benign and normal tissues,P<0.01. No relationship was found between the expression of telomerase activity and the clinical factors.CONCLUSION:Telomerase activation may contribute to the progression of ovarian carcinoma, and can probably become a promising target for gene therapy in ovarian carcinoma.