2Patel B K R,Banerjee D K,Butcher P D.Extraction and characterization of mRNA from mycobacteria:implication for virulence gene identification [J].J Micro Methods,1991,13:99～11.?A
3Mangan J A,Sole K M,Mitchison D A,et al.An effective method of RNA extraction from bacteria refractory to disruption,including mycobacteria [J].Nucleic Acids Res,1997,25:675～676.
4Lakey D L,Zhang Y,Talaat A M,et al.Priming reverse transcription with oligo (dT) does not yield representative samples of Mycobacterium tuberculosis cDNA [J].Microbiology,2002,148:2567～2572.
5Zhang J L,Byrne C D.Differential priming of RNA templates during cDNA systhesis markedly affects both accuracy and reproducibility of quantitative competitive reverse-transcriptase PCR [J].Biochem J,1999,337:231～241.
6Garbe T R,Hibler N S,Deretic V.Isoniazid induces expression of the antigen 85 complex in Mycobacterium tuberculosis [J].Antimicrob Agents Chemother,1996,40:1754～1756.
7Hellyer T J,Desjardin L E,Hehman G L,et al.Quantitative analysis of mRNA as a marker for viability of Mycobacterium tuberculosis [J].J Clin Microbiol,1999,37:290～295.?A?A
8Desjardin L E,Perkins M D, Wolski K,et al. Measurement of sputum Mycobacterium tuberculosis mRNA as a surrogate for response to chemotherapy [J]. Am Respir Crit Care Med, 1999,160:203 ~210.
9Rindi L, Lari N,Gil M G,et al. Oligo(dT)-primed synthesis of cDNA by reverse transcriptase in mycobacteria [J]. Biophy Res Commun, 1998,248:216 ~ 218.
10Belasco J G, Nilsson G,Von Gabain A,et al. The stability of E.coli gene transcripts is dependent on determinants localized to specific mRNA segments [J] .Cell, 1986,46:245 ~ 251.
9[1]Iovannisci D M,Winn-Deen E S.Ligation amplification and fluo rescence detection of Myeobacterium tuberculosis DNA[J].Mol Cell Probes,1993,7:35～43.
10[2]Hellyer T J,Desjardin L E,et al.Quantitative analysis of mRNA asa market for viability of Myeobactefium tuberculosis[J].J Clin Mierobiol.1999,37:290～295.
