抗生素对番茄愈伤组织诱导和分化的影响

Effect of antibiotics on the induction and differentiations of tomato callus

利用番茄子叶和下胚轴为材料,研究不同种类和浓度的抗生素对番茄愈伤组织诱导和生长的影响,及对农杆菌的抑菌效果。结果表明,在以卡那霉素作为抗性选择标记时,选择压力子叶为50mg·L-1,下胚轴为40mg·L-1;抑菌试验中,头孢霉素和羧苄青霉素的抑菌效果无明显差异,均能很好的抑制农杆菌生长,但头孢霉素对愈伤组织的不利影响大于羧苄青霉素。因此在番茄的遗传转化试验中,用羧苄青霉素的抑菌效果较好,建议使用浓度为350mg·L-1。

The effect of induction and differentiation of callus was studied with the cotyledon and hypocotyl of tomato in different kinds and concentration of antibiotics, and the effect of antibiotics on inhibiting Agrobacterium was also analyzed after infecting explants in tomato. Results indicated that the ideal selecting pressure of kanamycin was 50 mg·L-1 in cotyledon and 40 mg·L-1 in hypocotyls when kanamycin was used as selection marker. In the experiment of inhibiting Agrobacterium there were no significant differences between cefotaxime and carbenicillin. The negative effect of cefotaxime was more effective than that of carbenicillin. Therefore, the ideal antibiotics was carbenicillin and its ideal concentration was 350 mg·L-1 in tomato transformation.