灵菌红素对人胰腺癌肿瘤细胞增殖抑制的实验研究

Experimental studies on proliferation inhibitor of human pancreatic carcinoma cell of Prodigiosin

目的 证实灵菌红素对人胰腺癌细胞增殖有抑制作用。方法 活细胞能使外源性的MTT还原为难溶性的蓝紫色结晶物(Formazan)并沉积在细胞中,二甲基亚砜(DMSO)能溶解细胞中的蓝紫色结晶物,用酶联免疫检测仪在 4 90nm波长处测定其光吸收值,可间接反映活细胞数量。结果 灵菌红素IC50 值为 2 8μg·ml-1,5～ 4 0 μg·ml-1抑制效果明显。结论 提示灵菌红素对人胰腺癌细胞具有增殖抑制的作用。

Objective To investigate the act of prolif eration inhibitor of human pancreatic carcinoma cell of Prodigiosin.Method sLiving cells can deoxidise the external MTT to undissolve reddish-bl ue crystal and laid down.DMSO can disso l ve reddish-blue crystal in living cells,using enzyme immunoassay measuring ins t rument measure OD cost at 490 nm wavelength can infulection quantity of living cells.Results Prodigiosin IC 50 cost is 28mg·ml -1,obvious effect rate at the dose o f 5～40mg·ml -1.Conlusion The results indicated t ha t Prodigiosin possesses significant proliferating inhibition of human pancreatic carcinoma cell.