丹参对重症急性胰腺炎早期多器官组织脂质过氧化的影响

Effect of salvia miltiorrhiza on lipid peroxidation of multiple organs in rats with severe acute pancreatitis of early stage

[目的] 研究丹参对重症急性胰腺炎(SAP)早期多器官组织脂质过氧化的影响及其作用机制。[方法]将30只大鼠随机分为3组,即正常组、模型组和丹参治疗组。采用逆行胰管注射法建立大鼠 SAP模型,观察胰、心、肝、肺、肾组织的超氧化物歧化酶(SOD)、丙二醛(MDA)和组织学变化及丹参注射液对其的影响。[结果] 模型组的多器官组织MDA增加、SOD活性降低,以胰、心、肝、肾为主;丹参注射液可升高胰、心、肝、肾等组织的 SOD活性,降低胰、肝、肾组织的MDA水平。[结论] SAP早期存在着多器官组织脂质过氧化,相应组织中 SOD活性的降低是其原因之一。丹参可提高SAP多器官组织SOD活性而减轻组织脂质过氧化,以胰、肝、肾为主。

Objective] To investigate the mechanism of salvia miltiorrhiza in the early stage treatment of severe acute pancreatitis (SAP). [Methods] The levels of malondialdehyde (MDA), superoxide dismutase (SOD) and histological changes of pancreas, lungs, kidneys, liver and heart were measured after the SAP rats were induced by retrograde injection of 5% sodium taurocholate into hepatopancreatic duct. The changes of those indexes were also measured after salvia miltiorrhiza was injected intramuscularly just after induction of SAP. [Results] The levels of MDA in pancreatic, cardiac, hepatic, and renal tissue in SAP group were significantly higher than those in sham operation group, while the levels of SOD were significantly lower in the relevant tissues. The levels of MDA in pancreatic, hepatic and renal tissue in salvia miltiorrhiza group were significantly lower than those in SAP group; while the levels of SOD were significantly higher in the relevant tissues. [Conclusion] Lipid peroxidation in multiple organs occurs in the early stage of SAP, which is associated with the decrease of SOD level in the relevant tissues. Salvia miltiorrhiza may attenuate lipid peroxidation in multiple organs of SAP, especially in pancreas, liver and kidneys, and its effect of increasing SOD level in the relevant tissues may account for it.