化疗药物对荷人胆管癌裸小鼠移植瘤的药效试验

Effect of chemotherapeutic drugs on nude mice bearing human bile duct carcinoma

目的 :观察化疗药物对荷人胆管癌裸小鼠移植瘤的治疗效果。方法 :采用人中分化胆管癌裸小鼠移植瘤模型。将 4 5只荷人胆管癌裸小鼠分为各治疗组和对照组 ,裸小鼠治疗组每组 5只 ,对照组 10只。分组当天称鼠重 ,由尾静脉用药 1次。给药剂量 :丝裂霉素 (MMC) 4mg/kg ,阿霉素 (ADM) 10mg/kg ,长春新碱(VCR) 0 .2 0mg/kg ,泰素 (TAXOL) 2 0mg/kg,顺铂 (DDP) 10mg/kg ,环磷酰胺 (CTX) 12 0mg/kg ,5 氟脲嘧啶 ( 5 Fu) 12 0mg/kg ,NS为 0 .2ml/只。第 2 1天处死裸小鼠 ,称鼠重 ,计算体重变化。治疗期间每周测肿瘤瘤径 2次 ,计算相对肿瘤增殖率T/C( % )。结果 :MMC ,ADM ,VCR ,TAXOL ,DDP和CTX组第 2 1天相对肿瘤增殖率分别为 :1% ,2 3% ,39% ,4 7% ,5 1%和 86 % ,裸小鼠体重增加分别为 :2 1% ,0 % ,11% ,1% ,14 %和 8%。 5 Fu组80 %裸小鼠死亡 ,有药物毒性反应。结论 :MMC ,ADM ,VCR ,TAXOL ,DDP对荷人中分化胆管癌裸小鼠的移植瘤有明显的抗癌作用 。

Objective:To observe the effect of chemotherapeutic drugs on nude mice bearing human bile duct carcinoma.Methods:Human moderately differentiated bile duct carcinoma nude mouse model was used. 45 nude mice bearing human bile duct carcinoma were divided into 7 treated groups and one control group. Each treated group had 5 mice and the control group had 10 mice. On the day of classification,mouse weights were taken and drug was injected intravenously once via vena caudalis. The doses of drugs administrated were as follows:MMC 4 mg/kg,ADM 10 mg/kg,VCR 0.20 mg/kg,TAXOL 20 mg/kg,DDP 10 mg/kg,CTX 120 mg/kg,5-Fu 120 mg/kg. 0.2 ml of physiological saline solution was used for the control mouse. During the experiment,tumor sizes were measured twice a week and relative tumor proliferation rates were calculated. At the end of the experiment,mouse weights were measured again,changes of body weights were detected.Results:Relative tumor proliferation rates of MMC,ADM,VCR,TAXOL,DDP,CTX groups were 1%,23%,39%,47%,51% and 86%,respectively and increases of mouse body weights were 21%,0%,11%,1%,14% and 8%. 80% mice in 5-Fu group died during the experiment. It showed 5-Fu had toxic effect.Conclusion:MMC,ADM,VCR,TAXOL and DDP exhibit therapeutic effect on nude mice bearing human moderately differentiated bile duct carcinoma,however,CTX doesn't show such effect.