绝经后骨质疏松人群雌激素受体基因XbaⅠ多态性和年龄对骨密度的影响

Effect of Xba Ⅰ polymorphisms of estrogen receptor gene and age on bone mineral density in post-menopausal women

目的分析绝经后骨质疏松人群雌激素受体(ER)基因Xba Ⅰ多态性与骨密度的相关性以及在基因多态性下年龄、绝经年限及体重指数(BMI)对骨密度的影响。方法 用双能X线骨密度仪检测患者151例,以PCR-RFLP的方法检测ER基因Xba Ⅰ多态性,SPSS软件进行相关回归分析。结果 ER基因型的频率分布为XX型16,6％,Xx型67.5％,xx型15.9％,XX型的骨密度在腰椎、股骨颈和Ward's三角要高于Xx型和xx型,但ER多态性与骨密度无相关性。在Xx型和XX型人群中年龄越大,绝经年限越长,腰椎和股骨上端骨密度则越低。在XX型中,BMI是影响股骨颈和Ward's三角骨密度的主要因素。而在xx型中,腰椎和Ward's三角骨密度与年龄、BMI和绝经年限无相关性,绝经年限与股骨颈骨密度相关,大转子与BMI相关。结论福州地区绝经后骨质疏松人群ER基因Xba Ⅰ基因型与骨密度无明显相关性,但在不同基因型人群中,其骨密度的丢失趋势和影响因素也各异,临床上应采用不同的防治措施。

Objective To study the relationship between Xba I polymorphisms of the estrogen receptor (ER) gene and bone mineral density (BMD) and the effect of age, duration of menopause and body mass index (BMI)on BMD under different genotypes in postmenopausal women. Methods The BMD of 151 postmenopausal women were measured by dual energy X-ray absorptiometry. The polymorphisms of ER gene were detected by PCR-RFLP method. The correlation coefficients and stepwise regressions among the results were analyzed wsing SPSS software. Results The frequencies of ER genotypes of XX, Xx and xx were 16. 6% , 67. 5% and 15. 9% ,respectively. Although the BMD of lumbar spines, femur neck and Ward's region in XX were higher than in Xx and in xx, the correlations between the polymorphisms of ER gene and the BMD were not significantly different. In the Xx's and the XX's women, the older their ages and the longer their durations of menopause, the lower were their BMD . In the XX's patients, BMI was also the main factor affecting the BMD of femur neck and Ward's region. In the xx' patients, there were no correlations between the BMD of lumbar spines, Ward's region and age, BMI and duration of menopause, but there existed correlations between femur neck and duration of menopause, and between trochanter and BMI. Conclusions There is no correlation between the Xba I polymorphisms of ER gene and BMD, but the tendency of bone loss and influencing factors are different among patients of various genotypes'; hence we should take different measures for prevention and treatment of osteoporsis.