高压氧对鼠损伤脊髓内神经生长相关蛋白表达的影响

Effect of hyperbaric oxygen on the expression of GAP-43 in the spinal cord of rats underwent spinal cord injury

目的 研究高压氧 (HBO)治疗对脊髓损伤 (SCI)组织中神经生长相关蛋白 (GAP 43 )表达水平的影响。方法 用Allen氏WD法制作鼠脊髓损伤模型。 5 5只成年Wistar鼠随机分为正常组、SCI对照组和HBO治疗组。治疗组于术后每天HBO治疗 1次 ,对照组无特殊治疗。各组分别于术后 1d、4d、7d、10d、14d各随机取鼠 5只 ,取损伤脊髓组织 ,Westernblot法检测GAP 43表达水平。结果 GAP 43在正常成年鼠脊髓中少量表达 ,SCI后表达增加且HBO治疗组较对照组表达更为显著。术后 1周时SCI对照组GAP 43表达达到高峰 ,2周时降至接近初始水平 ,但HBO治疗组GAP 43表达仍维持高水平。结论 HBO可增强损伤脊髓组织GAP 43表达。

Objective To investigate the effect of hyperbaric oxygen (HBO) on expression of GAP 43 in the rats spinal cord following spinal cord injury (SCI). Methods Fifty five Wistar rats were randomly divided into three groups: a normal group, a control group and a HBO treatment group. The rats in the control and HBO treatment group underwent SCI by 10g×5cm impact at the T10 level. The rats in the HBO treatment group were treated by HBO. Western blot was used to observe the change of expression of GAP 43. Results There was weak expression of GAP 43 in the normal spinal cord. The GAP 43 expression increased significantly following SCI. The level of GAP 43 expression in the HBO treatment group was significantly high than that in the control group. Conclusion HBO can increase the level of GAP 43 expression.