电针对脑梗死大鼠神经前体细胞增殖水平的影响

The effect of electroacupuncture on proliferation of neural precursor cells in rats after acute cerebral infarction

目的 研究电针治疗对脑梗死病灶周围及海马处神经前体细胞增殖水平的影响。方法 采用易卒中型肾血管性高血压大鼠 (RHRSP) ,电凝法制成大脑中动脉闭塞 (MCAO)模型。行神经行为学功能评定、神经前体细胞标记及电针治疗 ,免疫组化染色观察并计数电针治疗后梗死灶边缘、对侧镜区及双侧海马5 溴脱氧尿核苷 (bromodeoxyuridine ,BrdU)标记的细胞。结果 MCAO后神经功能评分减低 ,约 5d后恢复正常。电针治疗促使梗死灶边缘、对侧镜区BrdU阳性细胞增多 ,随着治疗时间增加 ,细胞增多更明显。结论 电针治疗可促使神经前体细胞增殖及迁移 ,可能是电针促进康复疗效的重要机制之一。

Objective To investigate the distribution and proliferation of neural precursor cells(NPCs) in perifocal area and the hippocampus, and to examine the effect of electroacupuncture(EA) on them after acute cerebral infarction. Methods Stroke prone renovascular hypertensive rats(RHRSP) were used to establish middle cerebral artery occlusion(MCAO) models by electric coagulation. EA was applied 24 hour after cerebral infarction. Neurobehavioral tests and scoring were carried out once a day since MCAO, and twice a week the next week, until performing Garcia test before decapitation. The rats were decapitated 5 days and 2 weeks, respectively, after EA.The BrdU positive cells in ischemic perifocal area, contralateral mirror area and the hippocampus were checked by immunohistochemistry staining. Results The Garcia scores were less than 18 until the 5th day after MCAO,and the scores in EA group were significantly higher than that in the control group at the second and third days. There are more BrdU positive cells in the ipsilateral ischemic perifocal areas and hippocampus than that in the contralateral corresponding area(both EA group and control group, P <0.05). The NPCs congregated in the ischemic perifocal areas 2 weeks after MCAO ( P <0.01). BrdU positive cells increased significantly in the ischemic perifocal areas after EA treatment ( P <0.05), and increased as time went by within 2 weeks. Conclusion The proliferative NPCs in the ischemic perifocal areas and hippocampus can be induced by stroke and kept increasing during 2 weeks of the EA.Our data indicated that an endogeneous proliferative NPCs may play an important role in recovery from stroke.