肌动蛋白体外自组织复合纤维结构的观察

Observation on the Complex Structure of Actin Filaments Forming in Self-organization in vitro

利用原子力显微镜(atomicforcemicroscope,AFM)和透射电子显微镜(transmissionelectronmicroscope,TEM)技术,研究了低浓度肌动蛋白在体外简单热力学体系中,形成的自组织复合纤维结构。肌动蛋白在体外通过自组织过程能够聚合形成大尺度的、离散的、复杂的聚集纤维体系,分散的单根微丝较少;在微丝稳定剂鬼笔环肽干预下,肌动蛋白通过受调控的自装配过程,主要形成分散的单根微丝,以及少量由单根微丝组成的微丝束和纤维分支等简单微丝聚集结构。

The transmission electron microscope and the atomic force microscope-the novel tool for surface structure exploration, are employed to examine the structure of actin filaments forming in self-organization in vitro. It is observed that the actin can be polymerized into discrete and complicated filament complexes of tree-like branch structure and random coil filaments cluster in large scale in the simple thermodynamic solution without the addition of F-actin stabilizing reagents phalloidin. However in the presence of phalloidin, the polymerized filaments are mainly the single F-actin in dispersive distribution, and the intricate filaments made of microfilaments are seldom found under the same experimental conditions.